TY - JOUR ID - 107001 TI - Isolation, purification and identification of three diatom species (Bacillariophyceae) from Gomishan wetland (N. Iran) using phylogeny and silica cell wall ultra-structure analysis JO - Rostaniha JA - BOT.J.IRAN LA - en SN - 1608-4306 AU - Saba, Farkhondeh AU - Noroozi, Mostafa AU - Ghahremaninejad, Farrokh AU - Amoozegar, Mohammad Ali AU - Sedghi, Mahshid AU - Shahzadeh Fazeli, Seyed Abolhassan AU - Papizadeh, Moslem AD - MSc Graduate, Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran; Department of Plant Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran AD - Assistant Prof., Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran AD - Prof., Department of Plant Biology, Faculty of Biological Sciences, Kharazmi University, No. 43, Mofatteh Ave., Tehran 15719-14911, Iran (ghahremaninejad@khu.ac.ir) AD - Associate Prof., Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran; Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran, Iran AD - MSc Graduate, Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran, Iran AD - Associate Prof., Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran; Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran AD - PhD Student, Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran, Iran Y1 - 2016 PY - 2016 VL - 17 IS - 1 SP - 28 EP - 39 KW - Algae KW - Phytoplankton KW - pure culture KW - 18s rDNA DO - 10.22092/botany.2016.107001 N2 - Diatoms of Gomishan wetland (Golestan province, N. Iran), one of the most significant habitats in the eastern shore of the Caspian Sea, has been isolated, purified and identified during 2013. Using serial dilution and streaking on F/2 medium, pure, monoalgal and axenic cultures of the isolates were prepared. The isolates were characterized and identified using micro-morphological studies on the prepared permanent slides followed by scanning electron-microscopy. To prove the identification results, the most reliable genomic sequence fragments were investigated using GenBank database. Thus, SSU was amplified and analyzed, phylogenetically. The morphology and sequence data of three isolates were assessed which indicated that, the results of phylogenetic analyses of SSU-based sequences can support the morphological studies data. Finally, the isolates were introduced as Fallacia pygmaea, Halamphora coffeiformis andNavicula veneta. UR - https://rostaniha.areeo.ac.ir/article_107001.html L1 - https://rostaniha.areeo.ac.ir/article_107001_7f4952755790c5b33ba754ae25e5d38a.pdf ER -