TY - JOUR ID - 115958 TI - Molecular phylogenetic and morphometric evaluation of Calothrix sp. N42 and Scytonema sp. N11 JO - Rostaniha JA - BOT.J.IRAN LA - en SN - 1608-4306 AU - Nowruzi, Bahareh AU - Fahimi, Hossein AU - Ordodari, Neda AD - Assistant Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Assistant Prof., Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran AD - MSc Student, Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran Y1 - 2017 PY - 2017 VL - 18 IS - 2 SP - 210 EP - 221 KW - Calothrix sp. N42 KW - Nostocales KW - phylogenetic approaches KW - Scytonema sp. N11 KW - Taxonomy DO - 10.22092/botany.2018.115958 N2 - The taxonomy of cyanobacteria has been substantially modified in the last few decades, particularly after application of modern ultra structural and molecular methods. The present research, therefore, aimed to investigate, through a polyphasic approach, the differences in morphological and genotypic features of two cyanobacteria strains isolated from different agricultural and freshwaters areas in Kermanshah province (W Iran). In fact, identification and taxonomy of both Calothrix sp. N42 and Scytonema sp. N11 was problematic and confusing. Challenges arose when the two strains seemed to be Calothrix, because of early unbranched filaments, narrow cells and ending by heterocysts. Along with the progression of growth, branching arise. Therefore, molecular analysis was made and morphometric and genetic (16S rRNA) data were used to characterize the strains in liquid suspension cultures and solid media under photoautotrophic conditions. The results of sequencing the 16S rRNA fragment and phylogenetic comparison strongly indicated that, two strains fall into different phylogenetic clusters. Theywere named as Calothrix sp. N42 and Scytonema sp. N11 and have been registered under DDBJ, accession number KY548062 and KY548065, respectively.   UR - https://rostaniha.areeo.ac.ir/article_115958.html L1 - https://rostaniha.areeo.ac.ir/article_115958_f04c62a55b5e0dd20e8ddbc57db81e67.pdf ER -