New species of endophytic fungi from grapevine (Vitis vinifera) in Iran

Document Type : Research Paper

Authors

1 MSc Student, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Assistant Prof., Department of Plant Protection, Faculty of Agriculture, University of Urmia, Urmia, Iran

3 Prof., Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran (jnikkhah@ut.ac.ir)

4 Researcher, Sustainable Agro-Ecosystems and Bioresources Department, IASMA Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige, Italy

Abstract

During the study on endophytic fungi of grapevine (Vitis vinifera L.), 655 fungal isolates were obtained and studied taxonomically. Based on morphological and ITS sequences data, 15 species belonging to 10 genera including Alternaria brassicicola, A. chlamydospora, A. malorum, A. atra, Arthrinium phaeospermum, A. sacchari, Aspergillus nidulans, A. wentii, Beauveria bassiana, Cheatomium elatum, Epicoccum nigrum, Geosmithia pallida, Paecillomyces variotii, Cytospora punicae,and Verrucobotrys geranii are introduced as endophytic fungi of grapevine. Grapevineis as matrix nova for all mentioned species in Iran. Also, all identified species except A. phaeospermum, B. bassiana, and E. nigrum are reported for the first time as endophytic fungi of grapevine worldwide. Four species viz. A. sacchari, C. punicae, G. pallid, and V. geranii are introduced and described for the first time from Iran.

Keywords


Article Title [Persian]

معرفی گونه‌های جدیدی از قارچ‌های اندوفیت مو در ایران

Authors [Persian]

  • نازدار هرقلی 1
  • یوبرت قوستا 2
  • محمد جوان ‌نیکخواه 3
  • آندریا کامپیسانو 4
  • میشایل پانچر 4
1 دانشجوی کارشناسی ارشد گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
2 استادیار گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه
3 استاد گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
4 محقق گروه اکوسیستم کشاورزی و منابع زیستی پایدار، مرکز تحقیقات و نوآوری IASMA، ایتالیا
Abstract [Persian]

در این پژوهش، تعداد 655 جدایه اندوفیت قارچی از مو (Vitis vinifera L.) جداسازی شده و مورد مطالعه تاکسونومیکی قرار گرفتند. براساس مطالعات ریخت‌شناختی و توالی‌یابی ناحیه ITSاز DNAریبوزومی،15 گونه متعلق به 10 جنس شامل:Alternaria brassicicola، A. chlamydospora، A. malorum، A. atra، Arthrinium phaeospermum، A. sacchari،Aspergillus nidulans، A. wentii، Beauveria bassiana، Chaetomium elatum، Epicoccum nigrum، Geosmithia pallida، Paecilomyces variotii، Cytospora punicae و Verrucobotrys geranii به عنوان قارچ‌های اندوفیت از مو شناسایی و معرفی می‌شوند. انگور به عنوان میزبان جدید برای همه گونه‌های ذکر شده در ایران می‌باشد. همچنین، به غیر از گونه‌های A. phaeospermum، B. bassiana و E. nigrum، بقیه گونه‌های ذکر شده برای نخستین بار در دنیا به عنوان قارچ‌های اندوفیت از مو گزارش می‌شوند. چهار گونه شامل: A. sacchari، C. punicae،G. pallida و V. geranii برای نخستین بار از ایران گزارش و توصیف می‌شوند.

Keywords [Persian]

  • آرایه‌بندی
  • دی.ان.ای ریبوزومی
  • ریخت‌شناسی
  • قارچ‌ اندوفیت
Abdel-Motaal, F.F., Nassar, M.S.M., El-Zayat, S.A., El-Sayad, M.A. & Shin-Ichi, I. 2010. Antifungal activity of endophytic fungi isolated from Egyption henbane (Hyoscyamus muticus L.). Pakistan Journal of Botany 42: 2883–2894.
Amal, H.A., Debbab, A. & Proksch, P. 2011. Fungal endophytes: unique plant inhabitants with
great promises. Applied Microbiology and Biotechnology 90: 1820−1845.
Asgari, B., Zare, R. & Peyghami, E. 2004. Hyphomycetous fungal community of barley phylloplane in East Azarbaijan province with
 
 
 
 
emphasis on new taxa for Iranian fungal flora. Rostaniha 5: 67–70.
Brum, M.C.P., Araújo, W.L., Maki, C.S. & Azevedo, J.L. 2012. Endophytic fungi from Vitislabrusca L. (‘Niagara Rosada’) and its potential for the biological control of Fusarium oxysporum. Genetics and Molecular Research 11: 4187–4197.
Casieri, L., Hofstetter, V., Viret, O. & Gindro, K. 2009. Fungal communities living in the wood of different cultivars of Vitis vinifera plants. Phytopathologia Mediterranea 48: 73–83.
Cother, E.J. & Gilbert, R.L. 1994. The endophytic mycoflora of bladder saltbush (Atriplex vesicaria Hew. ex Benth.) and its possible role in the plant’s periodic decline. Proceedings of the Linnean Society of New South Wales 114: 149–169.
Crous, P.W. & Groenewald, J.Z. 2013. A phylogenetic re-evaluation of Arthrinium. IMA Fungus 4:
133–154.
Crous, P.W., Verldey, G.J.M., Groenewald, J.Z. & Samson, R.A. 2009. Fungal Biodiversity. CBS-KNAW Fungal Biodiversity Centre, Utrecht, the Netherlands, 269 pp.
Domsch, K.H., Gams, W. & Anderson, T.-H. 2007. Compendium of Soil Fungi. 2nd ed., IHW Verlag, Eching bei München, 672 pp.
Ellis, M.B. 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, England, 608 pp.
Ershad, D. 2009. Fungi of Iran. Third ed. Agricultural Research. Education & Extension Organization, Publication, Tehran, No. 10, 531 pp.
Eviner, V.T. & Chapin, F.S. 2003. Gopher-Plant-Fungal intractions affect establishment of an invasive grass. Ecology, 84: 120–128.
Fa´varo, L.C.L., Melo, F.L.D., Aguilar-Vildoso, C.I. & Arau´jo, W.L. 2011. Polyphasic analysis of intraspecific diversity in Epicoccum nigrum warrants reclassification into separate species. PLoS ONE 6(8): 1–18.
Ghosta, Y. 2004. Taxonomic study on Alternaria species in Iran. PhD thesis, Tarbiat Modares University, Tehran, Iran, 206 pp.
Giordano, L., Gonthier, P., Varese, G.C., Miserere, L. & Nicolotti, G. 2009. Mycobiota inhabiting sapwood of healthy and declining Scots pine (Pinus sylvestris L.) trees in the Alps. Fungal Diversity 38: 69–83.
González, V. & Tello, M.L. 2010. The endophytic mycota associated with Vitis vinifera in central Spain. Fungal Diversity 47: 29–42.
Gu, W. 2009. Bioactive metabolites from Alternaria brassicicola ML-P08; an endophytic fungus residing in Malus halliana. World Journal of Microbiology and Biotechnology 25:
1677–1683.
Hennebert, G.L. 1973. Botrytis and Botrytis-like genera. Persoonia 7(2): 183–204.
Houbraken, J., Verweij, P.E., Rijs, A.J.M., Borman, A.M. & Samson, R.A. 2010. Identification of Paecilomyces variotii in Clinical Samples and Settings. Journal of Clinical Microbiology 48(8): 2754–2761.
Hu, M.Y., Zhong, G.H., Sun, Zh.T. Shi, G., Liu, H.M. & Liu, X.Q. 2005. Insecticidal activities of secondary metabolites of endophytic Penicillium sp. in Derris elliptica Benth. Journal of Applied Entomology 129: 413–417.
Huang, H.C. & Erickson, R.S. 2007. Biological control of Sclerotinia stem rot of Canola using Ulocladium atrum. Plant Pathology Bulletin 16: 55–59.
Khan, M.R. 2007. Isolation, identification and cultivation of endophytic fungi from medicinal plants for the production and characterization of bioactive fungal metabolites. Ph.D. thesis, Department of Microbiology, University of Karachi, 246 pp.
Klich, M.A. & Pitt, J.I. 1988. A laboratory guide to the common Aspergillus species and their teleomorphs. Commonwealth Scientific and Industrial Research Organization, Division of Food Processing, Australia, 120 pp.
Klich, M.A. 2002. Identification of common Aspergillus species. Central voor Schimmeltures, Uterch, The Netherlands.116 pp.
Kolařík, M. & Kirkendall, L.R. 2010. Evidence for a new lineage of primary ambrosia fungi in Geosmithia Pitt ( Ascomycyta: Hypocreales). Fungal Biology 114: 676–689.
Kolařík, M., Kostovčík, M. & Pažoutová, S. 2007. Host range and diversity of the genus Geosmithia (Ascomycota: Hypocreales) living in association whit bark beetles in the Mediterranean area. Mycological Research 111: 1298–1310.
Kolařík, M., Kubátová, A., Pažoutová, S. & Šrůtka, P. 2004. Morphological and molecular characterization of Geosmithia putterillii,
G. pallida comb. nov. and G. flava sp. nov., associated whit subcorticolous insects. Mycological Research 108(9): 1053–1069.
Li, W.-C., Zhou, J., Guo, Y. & Guo, L.-D. 2007. Endophytic fungi associated with lichens in Baihua mountain of Beijing, China. Fungal Diversity 25: 69–80.
Liu, K., Ding, X., Deng, B. & Chen, W. 2009. Isolation and characterization of endophytic taxol-reducing fungi from Taxus chinensis. Microbiological Biotechnology 36: 1171–1177.
Maci´a-Vicente, J.G., Jansson, H.-B., Abdullah, S.K., Descals, E. & Lopez-Llorca, L.V. 2007. Fungal root endophytes from natural vegetation in Mediterranean environments with special reference to Fusarium spp. FEMS Microbiology Ecology 64: 90–105.
Mojtahedi, H., Rabie, C.J., Lubben, A., Steyn, M. & Danesh, D. 1979. Toxic Aspergillus from pistachio nuts. Mycopathologia 67: 123–127.
Mostert, L., Crous, P.W. & Petrini, O. 2000. Endophytic fungi associated with shoots and leaves of Vitis vinifera, with specific reference to the Phomopsis viticola complex. Sydowia 52: 46–58.
Novas, M.V. & Carmarán, C.C. 2008. Studies on diversity of foliar fungal endophytes of naturalized trees from Argentina, with a description of Haplotrichum  minutissimum sp. nov. Flora 203: 610–616.
Ownley, B.H., Gwinn, K.D. & Vega, F.E. 2010. Endophytic fungal entomopathogens whit activity aganinst plant pathogens: ecology and evolution. Biological Control 55: 113–128.
Pancher, M., Ceol, M., Corneo, P.E., Longa, C.M.O., Pertot, S.Y.I. & Campisano, A. 2012. Fungal endophytic communities in grapevines (Vitis vinifera L.) respond to crop management. Applied and Environmental Microbiology 78: 4308.
Pitt, J.I. & Hocking, A.D. 2009. Fungi and food spoilage. Springer, New York, 519 pp.
Puri, S.C., Verma, V., Amna, T., Qazi, G.N. & Spiteller, M. 2005. An endophytic fungus from Nothapodytes foetida that produces camptothecin. Journal of Natural Products 68: 1717–1719.
Qio, M., Xie, R.-S., Shi, Y., Zhang, H. & Chen, H.-M. 2010. Isolation and identification of two flavonoid prpducing endophytic fungi from Ginkgo biloba L. Annals of Microbiology 60: 143–150.
Rahimi, P., Sharifnabi, B. & Bahar, M. 2006. Aspergillus species isolated from pistachio and determination of their aflatoxin production. Rostaniha 8: 8–10.
Rehner, S.A., Minnis, A.M., Sung, G., Luangsa-ard, J.J., Devotto, L. & Humber, R.A. 2011. Phylogeny and systematics of the anamorphic entomopathogenic genus Beauveria. Mycologia 103: 1055–1073.
Sakayarij, J., Preedanon, S., Supaphon, O., Jones, E.B.G. & Phongpaichit, S. 2010. Phylogenetic diversity of endophyte assemblages associated with the tropical seagrass Enhalus acoroides in Thailand. Fungal Diversity 42: 27–45.
Saunders, M. 2010. Biotic filters in fungal endophyte community assembly. Ph. D. thesis. Department of Ecology and Evolutionary Biology, University of Toronto, 168 pp.
Şesan, T.E., Kohl, J. & Molhoeck, W.M.L. 2008. Ulocladium atrum Preuss-Biological control agent of grey mold (Botrytis cinerea Pers.) of cropped plants. PhD thesis, Institute of Plant
Diseases, Rheinischen Friedrich-Wilhelms-Universität Bonn, 140 pp.
Sette, L.D., Passarini, M.R.Z., Delarmelina, C., Salati, F. & Duarte, M.C.T. 2006. Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World Journal of Microbiology and Biotechnology 22: 1185–1195.
Shipunov, A., Newcombe, G., Raghavendra, A.K.H. & Anderson, C.L. 2008. Hidden diversity of endophytic fungi in an invasive plant. American Journal of Botany 95(9): 1096–1108.
Silhánová, M. & Novotny, D. 2006. Endophytes of branches and leaves of grapevine (Vitis vinifera L.) in the Czech Republic. 8th International Mycological Congress, Carins, Queensland, Australia, 322 pp.
Simmons, E.G. 2007. Alternaria an Identification Manual. CBS Biodiversity Series, No. 6. the Netherlands, 775 pp.
Sun, H.-F., Li, X.-M., Meng, L., Cui, C.-M., Gao, S.-S., Li, C.-S., Huang, C.-G. & Wang, B.-G. 2012. Asperolides A−C, tetranorlabdane diterpenoids from the marine algae-derived endophytic fungus Aspergillus wentii EN-48. Journal of Natural Products 75: 148–152.
Van, D.P. & Wachter, D.R. 1993. TREECON: a software package for the construction and drawing of evolutionary trees. Computer and Applied Bioscience 9: 177–182.
Vega, F.E., Posada, F., Aime, F.C., Pava-Ripoll, M. & Infante, F. 2008. Entomopathogenic fungal endophytes. Biological Control 46: 72–82.
Wang, Y.-T., Lo, H.-SH. & Wang, P.-H. 2008. Endophytic fungi from Taxus mairei in Taiwan: first report of Colletotrichum gloeosporioides as an endophytic of Taxus mairei. Botanical Studies 49: 39–43.
 
 
Weber, D. 2009. Endophytic fungi, Occurrence and Metabolites. Pp. 153–196. In: Esser, K. Anke, T. & Weber, D. (eds). The Mycota. Vol. 15: Physiology and Genetics Selected Basic and Applied Aspects. Springer, Germany.
White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M.A. Inis, D.H. Gelfand, J.J. Sninsky & T.J. White (eds). PCR Protocols: A guide to Methods and Applications. Academic Press, San Diego, U.S.A. pp. 315–322.
Wu, J., Ridgway, H.J., Carpenter, M.A. & Glare, T.R. 2008. Identification of novel genes associated whit conidiation in Beauveria bassiana with suppression subtractive hybridization. Mycologia 100: 20–30.
Zeinali, R. 2011. Study of genetic diversity of grapevine (Vitis vinifra L.) using ISSR genetic markr. MSc thesis. Urmia University, Urmia, Iran. 85 pp.