Molecular identification of basidiomycetous yeasts from soils in Iran

Document Type : Research Paper

Authors

1 PhD Student, Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran (mokhtarnejad@ut.ac.ir)

2 Associate Prof., Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

3 Prof., Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

4 Assistant Prof., Department of applied Microbiology; University if Perugia, Italy

Abstract

In the present study basidiomycetous yeast species were characterised from soil samples in different regions of Iran. Soil samples were collected from 5–15 cm depth, subsequently yeasts were isolated and purified. Yeast isolates were identified using D1/D2 domains of 26S rRNA and physiologic tests. The results of this study revealed a rich diversity amongst basidiomycetous yeast species in the sampled areas.  In this paper we report and characterise 25 species belonging to six genera viz., Trichosporon pullulans, Rhodotorula mucilaginosa, R. minuta, R. lysiniphila, R. laryngis, Rhodosporidium kratochvilovae, R. babjevae, Holtermanniella takashimae, Fibulobasidium inconspicuum, Crytococcus ramirezgomezianus, Cryptococcus watticus, C. victoriae, C. uzbekistanensis, C. terreus, C. saitoi, C. podzolicus, C. oeirensis, C. friedmannii, C. flavescens, C. diffluens, C. bhutanensis, C. albidus, C. albidosimilis, C. aerius, C. adeliensis. All of these species represent  new records for the mycobiota of Iran. Members of the genus Cryptococcus were the most donimnat basidiomycetous yeast and Cryptococcus aerius was commonly isolated from all soil samples as the dominat species.

Keywords


Article Title [Persian]

شناسایی مولکولی مخمرهای بازیدیومیستی در خاک‌های ایران

Authors [Persian]

  • لاچین مختارنژاد 1
  • مهدی ارزنلو 2
  • اسداله بابای اهری 3
  • بندتا تورکتی 4
1 دانشجوی دکتری بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشگاه تبریز، تبریز
2 دانشیار قارچ‌شناسی و بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشگاه تبریز، تبریز
3 استاد بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشگاه تبریز، تبریز
4 استادیار گروه میکروبیولوژی کاربردی، دانشگاه پروجیا، ایتالیا
Abstract [Persian]

در این مطالعه، تنوع زیستی مخمرهای بازیدیومیستی خاک­های نواحی مختلف ایران مورد برسی قرار گرفت. برای این منظور، نمونه‌های خاک از عمق 15-5 سانتی­متری جمع‌آوری و متعاقبا مخمرها جداسازی و خالص­سازی شدند. گروه­بندی اولیه جدایه­های مخمر از طریق انگشت‌نگاریDNA  با استفاده از MSP-PCR صورت گرفت. یک یا چند جدایه منتخب از هر گروه با استفاده از داده­های توالی نواحی D1/D2، ژن S rRNA26 و تست­های فیزیولوژیک شناسایی شدند. نتایج این مطالعه نشان داد که تنوع قابل توجهی در مخمرهای بازیدیومیستی خاک‌های مناطق نمونه‌برداری شده وجود دارد. در این مقاله، تعداد 25 گونه زیر متعلق به شش جنس نخستین بار برای مایکوبیوتای ایران معرفی می‌شوند: Trichosporon pullulans، Rhodotorul mucilaginosa، R. minuta، R. lysiniphila،R. laryngis، Rhodosporidium kratochvilovae، Rh. babjevae، Holtermanniella takashimae،Fibulobasidium inconspicuum  Cryptococcus ramirezgomezianus، C. watticus، C. victoriae، C. uzbekistanensis، C. terreus، C. saitoi، C. podzolicus،C. oeirensis، C. friedmannii، C. flavescens، C. diffluens، C. bhutanensis، C. albidus، C. albidosimilis،  aerius.C، adeliensis .C. در بین جنس­های شناسایی شده، Cryptococcusبیشترین فراوانی را به خود اختصاص داد وC. aerius به عنوان گونه غالب از تمام خاک­های نمونه­برداری شده جداسازی شد.

Keywords [Persian]

  • D1/D2
  • 26 rRNA gene
  • Cryptococcus
  • Rhodosporidium
  • Rhodotorula
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