New records of endophytic fungi isolated from oak trees in Kurdistan province (Iran)

Document Type: Research Paper

Authors

1 MSc Student of Plant Pathology, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

2 Associate Prof., Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran (jamini@uok.ac.ir)

3 Assistant Prof., Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

Abstract

Oak is the most important forest tree in Kurdistan province (Iran). Fungi are associated with oak trees as parasite, saprophyte and endophyte. The endophytic fungi are known components of biodiversity and have beneficial effects on host plants. During 2012-13 in a survey on endophytic fungi of oak trees in forest regions of Kurdistan province asymptomatic samples were collected from healthy twigs, stems and leaves. Isolation were made by plating pieces of samples, after washing with distilled water and surface sterilization with 70% ethanol, on Potato Dextrose Agar (PDA) supplemented with antibiotic. After incubation at 25o C under constant darkness for two weeks 67 isolates were obtained. Purification was made by transferring single spores to PDA plates. Based on morphological characters and ITS-rDNA sequence data, five species including Cladosporium tenellum, Paecilomyces formosus, Petriella guttulata, Preussia australis, and Sordaria sibutii were identified as new taxa for Iran mycobiota. All of these species are reported here as endophytic fungi from oak trees for the first time.

Keywords


Article Title [Persian]

معرفی آرایه‌های جدید از قارچ‌های اندوفیت بلوط در استان کردستان

Authors [Persian]

  • آسو حاجی‌زاده 1
  • جهانشیر امینی 2
  • جعفر عبداله‌زاده 3
1 دانش‌آموخته کارشناسی ارشد، بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشگاه کردستان، سنندج
2 دانشیار گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه کردستان، سنندج
3 استادیار گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه کردستان، سنندج
Abstract [Persian]

بلوط مهم‌ترین درخت جنگلی استان کردستان است. قارچ‌ها به صورت ساپروفیت، پارازیت و اندوفیت روی درختان بلوط زندگی می‌کنند. قارچ‌های اندوفیت بخشی از اجزای مهم تنوع زیستی مرتبط با گیاهان هستند و دارای اثرات مفید روی گیاهان میزبان خود هستند. به منظور شناسایی قارچ‌های اندوفیت از تنه، شاخه و برگ‌های سالم درختان بلوط در استان کردستان در بهار و تابستان سال‌های 91 و 92 نمونه‌برداری انجام گردید. نمونه‌ها در آزمایشگاه پس از شستشو با آب مقطر سترون به وسیله اتانول 70 درصد ضدعفونی و روی محیط سیب زمینی- دکستروز- آگار حاوی آنتی‌بیوتیک کشت و به مدت دو هفته در دمای 25 درجه سلسیوس نگهداری شدند. با استفاده از روش تک‌اسپور در مجموع 67 جدایه قارچ خالص به دست آمد. شناسایی گونه‌ها براساس خصوصیات ریخت‌شناختی و توالی ناحیه ITS-rDNA انجام گردید. بر این اساس، از این تعداد جدایه، پنج گونه به اسامی: Cladosporium tenellum، Paecilomyces formosus،Petriella guttulata،Preussia australis و Sordaria sibutii شناسایی شدند که آرایه‌های جدیدی برای مایکوفلور ایران هستند و برای نخستین بار به عنوان قارچ‌های اندوفیت درختان بلوط گزارش می‌شوند.

Keywords [Persian]

  • بلوط
  • قارچ اندوفیت
  • Paecilomyces
  • Petriella
  • Preussia
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