Introduction of some species of Parastagonospora on poaceous plants in Iran

Document Type: Research Paper


1 PhD Student, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

2 Prof., Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

3 Prof., Department of Plant Protection, University College of Agriculture and Natural Resources of Tehran University, Karaj, Iran


The main purpose of this study was to identify species of Parastagonospora in association with poaceous plants in Iran. Some species of Parastagonospora like P. nodorum causes a major loss to wheat production in the world. In the present research, two species including P. nodorum,and P. avenae were identified from different poaceous species. Parastagonospora nodorum was identifiedonPhalaris arundinacea. However, P. avenae was divided into two formaespecialsi.e. P. avenae f. sp. avenaria (Paa)onPhalaris arundinacea and Avena sativa, and the identified P. avenae
f. sp. tritici5 (Pat5) onPhalaris arundinacea, Bromus hordeaceus and Aegilops tauschii. Species identification was done according to morphology of anamorph and molecular confirmation was performed on the basis of ß-xylosidase gene sequence. Pat5 and Paa are reported from Iran for the first time and also all of the identified hosts are new to the world.


Article Title [Persian]

معرفی گونه‌هایی از جنس Parastagonospora روی گیاهان تیره گندمیان در ایران

Authors [Persian]

  • فریبا قادری 1
  • بهرام شریف نبی 2
  • محمد جوان نیکخواه 3
1 دانشجوی دکتری بیماری شناسی گیاهی، گروه گیاه‌پزشکی، دانشگاه صنعتی اصفهان، اصفهان، ایران
2 استاد گروه گیاه‌پزشکی، دانشگاه صنعتی اصفهان، اصفهان، ایران
3 استاد گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران
Abstract [Persian]

این مطالعه، با هدف شناسایی گونه‌های Parastagonospora مرتبط با گیاهان تیره گندمیان در ایران انجام شده است. برخی گونه‌های جنس ParastagonosporaمانندP. nodorum ، خسارت قابل ‌ملاحظه‌ای به این محصول در دنیا وارد می‌کنند. در این تحقیق، دو گونه از جنس Parastagonospora شامل P. nodorumوavenae  P. روی گندمیان شناسایی شدند. گونه
P. nodorum از روی Phalaris arundinacea شناسایی شد. گونهavenae  P.  نیز به دو گروه تقسیم شد که گروه اول آن،
P. avenae f. sp. avenaria (Paa) از روی Phalaris arundinaceaوAvena sativaوگروه دوم،P. avenae f. sp. tritici5 (Pat5) از روی Phalaris arundinacea، Bromus hordeaceusوAegilops tauschiiشناسایی شد. شناسایی این گونه‌ها براساس مشخصات ریخت‌شناسی مرحله غیرجنسی و تایید مولکولی آن‌ها براساس توالی‌یابی ژن بتا زایلوسیداز انجام گرفت. گروه‌های Pat5 و Paa برای نخستین بار از ایران گزارش می‌شوند و همچنین تمامی گندمیان مورد مطالعه در این تحقیق، به عنوان میزبان‌های جدید برای گونه‌های گزارش شده معرفی می‌شوند.

Keywords [Persian]

  • بتا زایلوسیداز
  • ریخت‌شناسی
  • سوختگی خوشه
  • فیلوژنی
  • گونه جدید
Aghajani, M.A., Kazemi, H., Dehghani, M.A., Rajaei, S. & Nuralahi, K. 2002. Study on distribution and importance of Septoria glum blotch of wheat in some provinces of Iran. Proceeding of the 1st International Wheat Congress, 7–10 Dec., Tehran, Iran: 37.

Arkadiusz, M., Chang, P.F., Zuzga, S.M., Chung, K.R., Shao, J. Cunfer, B.M., Arseniuk, E. & Ueng, P.P. 2006. RNA polymerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P. avenaria. Mycological Research 110(10): 1152–1164.

Bennett, R., Yun, S., Lee, T., Turgeon, B., Arseniuk, E., Cunfer, B. & Bergstrom, G. 2003. Identity and conservation of mating type genes in geographically diverse isolates of Phaeosphaeria nodorum. Fungal Genetic Biology 40(1): 25–37.

Cunfer, B.M. 2000. Stagonospora and Septoria diseases of barley, oat and rye. Canadian Journal of Plant Pathology 22(4): 332–348.

Cunfer, B. & Ueng, P. 1999. Taxonomy and identification of Septoria and Stagonospora species on small grain cereals. Annual Review of Phytopathology 37: 267–284.

DeWolf, E.D. & Francl, L.J. 2000. Neural network classification of tan spot and Stagonospora nodorum blotch infection periods in a wheat field environment. Phytopathology90(2): 108–113.

Ershad, D. 2009. Fungi of Iran. 3rd ed. Iranian Research Institute of Plant Protection, Tehran, 531 pp.

Eyal, Z., Scharen, A.L., Prescott, J.M. & Van Ginkel, M. 1987. The Septoria disease of wheat: concepts and methods of disease management. CIMMYT Mexico, D.F.

Gaunt, R.E., Thompson, W.J. & Sutcliffe, J. 1986. The assessment of speckled leaf blotch in winter wheat in New Zealand. Annals of Botany 58: 33–38.

Ghaderi, F., Sharifnabi, B. & Javan-Nikkhah, M. 2016. Assessment of partial resistance of wheat cultivars to Phaeosphaeria nodorum in Iran. Journal of Applied Entomology and Phytopathology 84(1): 55–66.

Halama, P. & Lacoste, L. 1992. Déterminisme de la reproduction sexuéedu Phaeosphaeria (Leptosphaeria) nodorum agent de la septoriose du blé. II. Action de la température et de la lumière. Canadian Journal of Botany 70(8): 1563–1569.

Halama, P., Skajennikoff, M. & Dehorter, B. 1999. Tetrad analysis of mating type, mutations, esterase and aggressiveness in Phaeosphaeria nodorum. Mycological Research 103(1): 43–49.

Malkus, A., Reszka, E., Chang, C.J., Arseniuk, E., Chang, P.F.L. & Ueng, P.P. 2005. Sequence diversity of b-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria. FEMS Microbiology Letters 249(1): 49–56.

Miyake, I. 1909. Studies on the parasitic fungi of rice in Japan. Botical Magazine Tokyo 23: 85–97.

Katoh, K. & Standley, D.M. 2013. MAFFT multiple sequence alignment software. Version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4): 772–780.

McDonald, M.C., Razavi, M., Friesen, T.L., Brunner, P.C. & McDonald, B.A. 2012. Phylogenetic and population genetic analysis of Phaeosphaeria nodorum and its close relative indicate cryptic species and an origin in the Fertile Crescent. Fungal Genetic Biology 49(9): 882–895.

McFadden, W. & Harding, H. 1989. Cereal stubble as a source of primary inoculum of leaf-spotting pathogens of winter wheat. Canadian Journal of Plant Pathology11(2): 195 (Abstr.).

Murray, M. & Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8(19): 4321–4325.

Norvel, L.L. 2011. Fungal nomenclature. 1. Melbourne approves a new Code. Mycotaxon 116(1): 481–490.

Quaedvlieg, W., Kema, G.H.J., Groenewald, J.Z., Verkley, G.J.M., Seifbarghi, S., Razavi, M., Mirzadi Gohari, A., Mehrabi, R. & Crous, P.W. 2011. Zymoseptoria gen. nov.: a new genus to accommodate Septoria-like species occurring on graminicolous hosts. Persoonia 26: 57–69.

Quaedvlieg, W., Verkley, G.J.M., Shin, H.D., Barreto, R.W., Alfenas, A.C., Swart, W.J., Groenewald, J.Z. & Crous, P.W. 2013. Sizing up Septoria. Studies in Mycology 75(1): 307–390.

Reszka, E., Chung, K.R., Tekauz, A., Malkus, A., Arseniuk, E., Krupinsky, J.M., Tsang, H. & Ueng, P.P. 2005. Presence of B-glucosidase (bgl1) gene in Phaeosphaeria nodorum and Phaeosphaeria avenaria f. sp. tritici. CanadianJournal of Botany 83(8): 1001–1014.

Saitou, N. & Nei, M. 1987. The Neighbor-Joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4(4): 406–425.

Shaw, D. 1957. Studies on Leptosphaeria avenaria f. sp. tritici on cereals and grasses.CanadianJournal of Botany 35(1): 113–118.

Shoemaker, R.A. & Babcock, C.E. 1989. Phaeosphaeria. Canadian Journal of Botany 67(5): 1500–1599.

Solomon, P.S., Lowe, R.G.T., Tan, K.C., Waters, O.D.C. & Oliver, R.P. 2006. Stagonospora nodorum: cause of Stagonospora nodorum blotch of wheat. Molecular Plant Pathology 7(3): 147–156.

Sommerhalder, R.J., McDonald, B.A. & Zhan, J. 2006. The frequencies and spatial distribution of mating types in Stagonospora nodorum are consistent with recurring sexual reproduction. Phytopathology96(3): 234–239.

Sprague, R. 1950. Disease of Cereal and Grasses in North America. Ronald Press, New York. 538 pp.

Tamura, K., Dudley, J., Nei, M. & Kumar, S. 2007. Molecular Evolutionary Genetics Analysis (MEGA). Software Version 4.0. Molecular Biology and Evolution 24(8): 1596–1599.

Ueng, P., Cunfer, B., Alano, A., Youmans, J. & Chen, W. 1995. Correlation between molecular and biological characters in identifying the wheat and barley biotypes of Stagonospora nodorum. Phytopathology 85(1): 44–52.

Ueng, P., Dai, Q., Cui, K.-R., Czembor, P.C., Cunfer, B.M., Tsang, H., Arseniuk, E. & Bergstrom, G.C. 2003. Sequence diversity of mating-type genes in Phaeosphaeria avenaria. Current Genetics 43: 121–130.

Ueng, P., Subramaniam, K., Chen, W., Arseniuk, E., Wang, L., Cheung, A., Hoffmann, G. & Bergstrom, G. 1998. Intraspecific genetic variation of Stagonospora avenae and its differentiation from S. nodorum. Mycological Research 102(5): 607–614.

 Zhang, Y., Schoch, C.L., Fournier, J., Crous, P.W., de Gruyter, J., Woudenberg, J.H.C., Hirayama, K., Tanaka, K., Pointing, S.B., Spatafora, J.W. & Hyde, K.D. 2009. Multi-locus phylogeny of Pleosporales: a taxonomic, ecological and evolutionary re-evaluation. Studies in Mycology 64: 85–102.

Wang, C.L., Arkadiusz, M., Zuzga, S.M., Chang, P.F.L., Cunfer, B.M., Arseniuk, E. & Ueng, P.P. 2007. Diversity of the trifunctional histidine biosynthesis gene (his) in cereal Phaeosphaeria species. Genome 50(3): 595–609.

Weber, G.F. 1922. Septoria diseases of wheat. Phytopathology 12(12): 537–585.

Wingfield, M.J., de Beer, Z.W., Slippers, B., Wingfield, B.D., Groenewald, J.Z., Lombard, L. & Crous, P.W. 2012. One fungus, one name promotes progressive plant pathology. Molecular Plant Pathology 13(6): 604–613.