Micromorphological study of the tribe Agrimonieae family Rosaceae in Iran

Document Type: Research Paper


1 Assistant Prof., Department of Biology, Faculty of Science, University of Gilan, Rasht, Iran

2 MSc Student, Department of Biology, Faculty of Science, University of Gilan, Rasht, Iran


The current study, devoted to leaf micromorphology of tribe Agrimonieae in Iran including three genera (Agrimonia, Aremonia,and Sanguisorba),four species (Agrimonia eupatoria,Aremonia agrimonioides,S. minor, and
S. officinalis), and six subspecies (A. eupatoria subsp. eupatoria, A. eupatoria subsp. grandis, A. eupatoria subsp. asiatica, S. minor subsp. minor, S. minor subsp. lasiocarpa, and S. minor subsp. muricata). At first, plant materials were collected and identified and then leaf segments were washed and prepared for Scanning Electron Microscopy (SEM). The result of micromorphological analysis revealed three types of trichomes (curved, flexuous and straight), four types of hair surface ornamentations (echinate, transversely elongated papilla, verucate and granulate), and two types of glandular hairs (round to cylindrical head cell with pisilate to echinate surfaces). In all the examined species, epicuticulare wax types were either of film (smooth layers and crust), and crystalloids (granule and platelets), and wax sculpturing which comprises four types of syntupism. In addition, stomata traits were identified especially three types of outerstomatal rim/peristomatal rim, four types of inner stomata rim, and three types of wax distribution on the stomata rim/pore/epidermal cell. Based on author’s achievements, micromorphological characters of studied species of tribe Agrimonieae, possess diagnostic value and were capable of separating different taxonomic ranks (subtribes, genera, species, and subspecies). Based on these characters, an identification key is also prepared and presented herewith.


Article Title [Persian]

مطالعه ریزریخت‌شناسی برگ طایفه Agrimonieae از تیره‌ گل‌سرخیان در ایران

Authors [Persian]

  • مرضبه بیگم فقیر 1
  • گلنوش شمشیری 2
  • عاطفه مهرمنش 2
1 استادیار گروه زیست‌شناسی، دانشکده علوم، دانشگاه گیلان، رشت، ایران
2 دانشجوی کارشناسی ارشد، گروه زیست‌شناسی، دانشکده علوم، دانشگاه گیلان، رشت، ایران
Abstract [Persian]

مطالعه حاضر به مطالعه صفات ریزریخت‌شناسی برگ گیاهان طایفهAgrimonieae  (syn.: Sanguisorbeae DC.)، شامل سه جنس (Agrimonia، AremoniaوSanguisorba)، چهار گونه (Agrimonia eupatoria، Aremonia agrimonioides،S. minor و officinalisS.)، شش زیرگونه (A. eupatoria subsp. eupatoria، A. eupatoria subsp. grandis، A. eupatoria subsp. asiatica،
S. minor subsp. minor، S. minor subsp. lasiocarpa و S. minor subsp. muricata) می‌پردازد. ابتدا نمونه‌های گیاهی جمع‌آوری و شناسایی و سپس قطعات برگ شستشو و برای عکسبرداری توسط میکروسکوپ الکترونی  (SEM)آماده شدند. نتایج حاصل از آنالیز ریزریخت‌شناسی، سه نوع کرک (خمیده، موجدار و راست)، چهار نوع تزیینات سطح کرک (خار مانند، پشته‌های عرضی، زگیل‌دار و گرانول‌دار، دو نوع کرک غده‌ای (سر گرد و استوانه، با سطح صاف تا خاردار)، دو نوع موم روی کوتیکولی لایه نازک (لایه‌ای صاف و پوسته پوسته) و کریستالوییدی (گرانول‌دار، صفحات کوچک) و چهار الگوی ترکیبی تزیینات موم را آشکار ساخت شد. به علاوه، در این مطالعه، صفات روزنه بویژه سه نوع الگوی لبه‌ بیرونی/تقریبا درونی و چهار نوع لبه‌ درونی روزنه و سه الگوی پراکنش موم روی لبه درونی روزنه/سلو‌ل‌های منفذ/سلول‌های نگهبان شناسایی شدند. براساس یافته‌های این تحقیق، صفات ریز‌ریخت‌شناسی گونه‌های مورد بررسی طایفه‌ Agrimonieaeحاوی اطلاعات تشخیصی و برای جداسازی سطوح مختلف تاکسونومیک (زیرطایفه، جنس، گونه‌ها و زیر‌گونه‌ها) کارآمد می‌باشند. براساس این صفات، کلید شناسایی نیز تهیه و ارایه گردید.

Keywords [Persian]

  • پهنک برگ
  • SEM
  • کرک
  • موم روی کوتیکولی
  • Agrimonia؛ Aremonia؛ Sanguisorba
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