Molecular and micro-morphological evidences of the genus Cuscuta in Iran

Document Type : Systematics and Biodiversity of Plants

Authors

1 PhD Graduate, Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

2 PhD Graduate, Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran

3 Associate Prof., Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

4 MSc Graduate, Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

5 Assistant Prof., Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

Abstract

Cuscuta is the only parasitic genus in Convolvulaceae family. This genus is globally distributed, with most species in the tropics, subtropics, and some in the temperate regions. In this study, the micro-morphological features and molecular evidencesof 12 populations from three species of Cuscuta (C. australis, C. campestris,and C. chinensis) have been considered. In total, seven quantitative and two qualitative characters of pollen were selected and measured. The most important characters include: shape, ornamentation of tectum, exine thickness and colpus length of the pollen. Based on this study, the seed shape and surface support at least for separation of C. australis from other two species. Using nuclear (nrDNA ITS) marker, we reconstructed phylogenetic relationships within three species of Cuscuta. This data set was analyzed by phylogenetic methods including Bayesian, Maximum likelihood, and Maximum parsimony. In phylogenetic analyses, all members of three species formed a well-supported clade (PP=1, ML/BS=100/100) and divided into two major clades (A and B). Clade A is composed of specimens of C. australis. Two species of C. campestris and C. chinensis are nested in clade B. Neighbor-Net diagram demonstrated separation of the studied populations.
The results showed that, micro-morphological and molecular data provide reliable evidence for separation of these species.
 

Keywords

Main Subjects

Article Title [Persian]

شواهد مولکولی و ریزریخت‌شناختی جنس سس در ایران

Authors [Persian]

  • الهام امینی 1
  • فاطمه نصر الهی 2
  • علی ستاریان 3
  • سهیلا کر 4
  • سهراب بوذرپور 5
1 دانش آموخته دکتری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران
2 دانش آموخته دکتری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه قم، قم، ایران
3 دانشیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران
4 دانش موخته کارشناسی ارشد، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران
5 استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران
Abstract [Persian]

سس (Cuscuta) تنها جنس انگلی در تیره پیچکیان است. این جنس پراکنش جهانی داشته و اکثر گونه‌های آن در مناطق گرمسیری، نیمه‌گرمسیری و بعضی در مناطق معتدل پراکندگی دارند. در این مطالعه، ویژگی‌های ریزریخت‌شناختی و مولکولی 12 جمعیت از سه گونه سس (C. australis،
C. campestris و C. chinensis) مورد بررسی قرار گرفت تا ارزش تشخیصی آن‌ها ارزیابی شود. در کل، هفت ویژگی کمی و دو ویژگی کیفی در گرده انتخاب شده و مورد اندازه‌گیری قرار گرفتند. مهمترین ویژگی‌ها شامل شکل، تزیینات تکتوم، ضخامت اگزین و طول شیار گرده هستند. براساس این تحقیق، شکل و سطح دانه، جدایی C. australis را از دو گونه دیگر مورد تایید قرار داد. همچنین در این تحقیق، با استفاده از نشانگر هسته‌ای (nrDNA ITS)، روابط فیلوژنتیکی در سه گونه از این آرایه بازسازی گردید. داده‌ها توسط آنالیزهای فیلوژنتیکی شامل بیزین، بیشینه درست‌‌نمایی و بیشینه صرفه‌جویی مورد بررسی قرار گرفتند. در آنالیزهای فیلوژنتیکی همه اعضای این سه گونه یک شاخه با حمایت بسیار بالا (PP=1, ML/BS=100/100) تشکیل دادند و به دو گروه اصلی تفکیک شدند (A و B). شاخه A از نمونه‌های گونه C. australis تشکیل شده و دو گونه  C. campestrisو C. chinensis در شاخه B قرار گرفتند. روش شبکه-همسایه، جدایی جمعیت‌های مورد مطالعه را ثابت کرد و نتایج نشان داد که داده‌های ریزریخت‌شناختی و مولکولی، جدایی این گونه‌ها را از هم تایید می‌کنند.

Keywords [Persian]

  • پیچکیان
  • دانه
  • شبکه-همسایه
  • فاصله‌گذار رونویسی شونده درون هسته‌ای
  • گرده

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Rostaniha
Volume 19, Issue 2 - Serial Number 56
December 2018
Pages 113-129

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History

  • Receive Date: 31 July 2018
  • Revise Date: 19 August 2018
  • Accept Date: 01 December 2018

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