Genetic diversity of Athelia rolfsii populations in the north of Iran

Document Type : Systematics and Biodiversity of Fungi

Authors

1 PhD Student, Department of Plant Protection, College of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate Prof., Department of Plant Protection, College of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Associate Prof., Department of Biotechnology, College of Agricultural Sciences, University of Guilan, Rasht, Iran

4 Assistant Prof., Department of Plant Protection, College of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

Athelia rolfsii is a globally distributed soil-borne fungal pathogen causing root rot disease in many crops. In order to study the genetic diversity of this fungus, 90 isolates were collected and isolated from Guilan, Mazandaran and Golestan provinces (North of Iran). Genetic diversity of these isolates was investigated using Mycelial Compatibility Groups tests (MCG) and ISSR and SCoT markers. In this study, nine MCG groups were identified. The MCG3 group (with 36 members) was the most frequent in terms of the number of isolates and the most diverse group in terms of host domain (due to infection of eight host species). This group (MCG3) was also the only group that isolated from specimens in all three provinces. The dendrogram derived from cluster analysis of ISSR primers divided the isolates into four groups at a similarity level of 57%. The dendrogram obtained from cluster analysis of SCoT primers placed the isolates in a similarity level of 59% in three groups. Based on the results of both markers, Golestan isolates (except two isolates) were separated from Guilan and Mazandaran isolates. However, the presence of these two isolates in a separate cluster along with the isolates of Guilan and Mazandaran provinces was consistent with the results of MCG grouping. The results of this study showed that, these two types of markers are useful to differentiate isolates based on geographic regions.

 
 
 

Keywords

Article Title [Persian]

تنوع ژنتیکی جمعیت‌های قارچ Athelia rolfsii در شمال کشور

Authors [Persian]

  • زهرا مهری 1
  • سید اکبر خداپرست 2
  • علی اعلمی 3
  • صدیقه موسی نژاد 4
1 دانشجوی دکتری بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
2 دانشیار گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
3 دانشیار گروه بیوتکنولوژی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
4 استادیار گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
Abstract [Persian]

گونهAthelia rolfsii ، یک قارچ بیماری‌زای خاک‌زی با پراکنش جهانی است که به عنوان عامل بیماری پوسیدگی سفید ریشه در بسیاری از گیاهان زراعی و باغی شناخته می‌شود. به منظور بررسی تنوع ژنتیکی این قارچ، تعداد 90 جدایه، از سه استان گیلان، مازندران و گلستان جمع‌آوری و جداسازی شدند و سپس با استفاده از گروه‌های سازگار میسلیومی (MCG) و دو نشانگر مولکولی ISSR و SCoT مورد مطالعه قرار گرفتند. در این بررسی، نه گروه سازگار میسلیومی شناسایی شد. گروه سازگار میسلیومی MCG3 (با 36 عضو) دارای بیش‌ترین فراوانی از نظر تعداد جدایه و متنوع‌ترین گروه از لحاظ دامنه میزبانی (با آلودگی هشت گونه گیاهی میزبان) بود. همچنین، این گروه تنها گروهی بود که از نمونه‌های هر سه استان جداسازی شده است. دندروگرام حاصل از تجزیه خوشه‌ای آغازگرهایISSR ، جدایه‌ها را در سطح تشابه 57% به چهار گروه تقسیم کرد. دندروگرام حاصل از تجزیه خوشه‌ای آغازگرهایSCoT ، جدایه‌ها را در سطح تشابه 59% در سه گروه قرار داد. نتایج حاصل از تجزیه به مختصات اصلی نیز این گروه‌بندی‌ها را تایید کرد. براساس نتایج هر دو نشانگر، جدایه‌های استان گلستان (به جز دو جدایه) از جدایه‌های گیلان و مازندران تفکیک شدند. قرار گرفتن این دو جدایه در یک کلاستر جداگانه در کنار جدایه‌های گیلان و مازندران، با نتایج حاصل از گروه‌بندی MCG مطابقت داشت. نتایج این بررسی نشان داد که این دو نوع نشانگر، توانایی تفکیک جدایه‌ها را براساس مناطق جغرافیایی دارا می‌باشند.
 
 

Keywords [Persian]

  • پوسیدگی سفید
  • نشانگر مولکولی
  • ISSR
  • MCG
  • SCoT

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Rostaniha
Volume 21, Issue 1 - Serial Number 59
September 2020
Pages 14-26

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History

  • Receive Date: 07 January 2020
  • Revise Date: 16 March 2020
  • Accept Date: 08 April 2020

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